ezFlow Endofree Plasmid Maxiprep-25Units

Key to the plasmid purification kit is our proprietary DNA binding system that allows the high efficient binding of DNA to our ezBindTM matrix while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with sterile water or Elution Buffer. Unlike other kits in the markets, our patented plasmid purification kit has no chaotropic salts in the buffer. The purified DNA is guanidine/anion exchange resin residues free. The EZgeneTM endofree system uses a specially formulated buffer that extracts the endotoxin from the bacterial lysate. The endotoxin level is less than 0.1 EU (Endotoxin) per µg of plasmid DNA. This kit is designed for fast and efficient purification of plasmid DNA from 150 to 200 mL of E. coli culture. The maxi column has a DNA binding capacity of 1200 µg. The purified endofree DNA is ready for downstream applications such as transfection of endotoxin-sensitive cell lines, primary cultured cells or microinjection. Two endotoxin removal procedures are provided. Protocol A removes endotoxin during the purification of plasmid DNA and Protocol B removes endotoxin after the purification of plasmid DNA.