Insect gDNA Kit -4Units

Product Description Biomiga Insect Genomic DNA Kit, 4 Preps, Each ezBind DNA column can bind approximately 100ug DNA. Using greater than 30mg tissue is not recommended, Yield up to 60kb, Includes Buffer BL, ITL, KB, Proteinase K, RNase A, DNA Wash Buffer, Elution Buffer, ezBind DNA Mini Columns (50), 2mL Collection Tubes (100), User Manual. Details Insect gDNA Isolation Kits: is designed for efficient recovery of genomic DNA up to 60 kb in size from insects, arthropods, and some plant tissue samples rich in polysaccharides. The method is suitable for samples frozen or preserved in alcohol or DNE solution, and good results can be obtained with formalin preserved material. Samples are homogenized and lysed in a high salt buffer and extracted with chloroform to remove polysaccharides. Following a rapid alcohol precipitation step, binding conditions are adjusted and DNA further purified using ezBindTM DNA spin columns. In this way, salts, proteins and other contaminants are removed to yield high quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.