Mullusc gDNA Kit -250Units

Product Description Biomiga Mullusc gDNA Kit, 250 Preps, Each ezBind DNA column can bind approximately 100ug DNA. Using greater than 30mg tissue is not recommended, Yield up to 60kb, Includes Buffer MBL, MTL, KB, Proteinase K, RNase A, DNA Wash Buffer, Elution Buffer, ezBind DNA Mini Columns (50), 2mL Collection Tubes (100), User Manual. Details Mollusc gDNA Isolation Kits: is designed to extract genomic DNA up to 60 kb in size from molluscs, insects, arthropods, roundworms, flatworms, and other invertebrate tissue samples rich in mucopolysaccharides. The method is suitable for invertebrates frozen or preserved in alcohol or DNE solution, and good results can be obtained with formalin preserved material. Samples are homogenized and lysed in a high salt buffer and extracted with chloroform to remove mucopolysaccharides. Following a rapid alcohol precipitation step, binding conditions are adjusted and DNA further purified using ezBind DNA spin columns. While proteins and other contaminants are removed by wash buffer, high quality genomic DNA is eluted with elution buffer or sterile water. The purified genomic DNA is suitable for downstream applications such as Southern Blot, restriction digestion, and PCR.