One-Step RT-PCR Kit-25Units

One-Step RT-PCR is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA. This system uses a mixture of MMLV Reverse Transcriptase and Hot start Taq DNA polymerase in an optimized reaction buffer, and can detect RNA targets up to 4.0 kb. The amount of input total RNA can range from 0.01 pg to 2 μg. Sufficient reagents are provided for 100 amplification reactions of 50 μl each.