COVIDelta Detection Kit

With Biomiga's proprietary specific primer design technology, this kit detects simultaneously COVID RdRp gene and Delta (India, B.1.617.2) variant (with two set of primers against two point-mutations to ensure accuracy). The primers and probes are selected from the regions of RNase P, ORF1ab and S gene. Three primer/probe sets are designed for specific detection of SARS-CoV-2 and delta mutations in S gene. An additional primer/probe set to detect human RNase P gene (as an internal control) is also included. RNA extracted from respiratory specimens is reverse transcribed and amplified in a Real-Time PCR Thermocycler (such as Applied Biosystems 7500 Real-Time PCR Instrument). The fluorescent signal is emitted when the 5’ nuclease active domain of Taq polymerase degrades the probe anneals to target sequence between the forward and the reverse primers. During each cycle, the fluorescence intensity is increased with the additional reporter dye molecules released from their respective probes.