One Step qRT-PCR 2x Mix 12.5 mL

Over 5 million reactions shipped for COVID-19 RT-qPCR detection The One-Step qRT-PCR System, No ROX or Low Rox is consist of Enzyme Mix and 2X Reaction Mix, It is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA in a single tube for the detection and quantification of RNA using real-time detection instruments. The proprietary Enzyme Mix contains an engineered Script Reverse Transcriptase (RNaseH minus), Hot Start Taq DNA polymerase, stabilizers. 2X Reaction Mix consists of a proprietary buffer system, 6 mM MgCl2, dNTPs, enhancers and stabilizers. It also contains an optimal concentration of ROX for instruments that require low ROX for a reference signal. Please consult our website and Instrument Compatibility section below for the correct product for your real-time PCR system. Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to block polymerase activity between ambient to RT reaction temperature. Script RT enzyme can synthesize cDNA at a temperature range of 40-55oC. Once the PCR step reaches denaturation temperature (95oC), Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield. The system enables highly sensitive detection from as few as 10 copies of SARS-CoV-2 target genes (N1 and N2), with a broad dynamic range that supports accurate quantification of mRNA copies. Sufficient reagents are provided for 125, 500, or 1,250 amplification reactions of 20 μl each. Features: Easy-to-use two tube system: Enzyme Mix (50X) and Reaction Mix (2X) Broad range of RT reaction temperature (40-55oC) Broad dynamic range of detection Hot start PCR system Fully optimized RT-qPCR buffer and robustness High sensitivity, specificity, and reproducibility Ideal for probe based COVID-19 viral RNA detection and quantitation